Plasmid_Backbone
pSB8K15

Part:BBa_K864018:Design

Designed by: Erik Lundin   Group: iGEM12_Uppsala_University   (2012-09-24)


Low copy BioBrick temperature sensitive standard vector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3642
    Illegal NheI site found at 2425
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3642
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3642
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3642
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3657
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.

The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.

The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.


Source

Based on BBa_K864002

References